You are in:Home/Publications/Impact of in vitro fertilization by fresh and frozen semen on developmental competence and cryotolerance of buffalo embryos

Dr. Ahmed Reda Mohamed Hassan El-Khawagah :: Publications:

Title:
Impact of in vitro fertilization by fresh and frozen semen on developmental competence and cryotolerance of buffalo embryos
Authors: Soliman, W. T. M.1; Mahmoud, K. Gh. M.2*; El-Khawagah, A. R. M.3; Kandiel, M. M. M.3; Abouel-Roos, M. E. A.3; Abdel-Ghaffar, A. E.3 and El Azab, A. E. I.3
Year: 2018
Keywords: Buffalo, Embryo vitrification, Fresh and frozen semen, In vitro fertilization
Journal: Iranian Journal of Veterinary Research
Volume: 19
Issue: 3
Pages: 178-181
Publisher: IJVR
Local/International: International
Paper Link:
Full paper Ahmed Reda Mohamed Hassan El-Khawaga_IJVR_Volume 19_Issue 3_Pages 178-181.pdf
Supplementary materials Not Available
Abstract:

Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen in fertilization on developmental competence and cryo-survival of buffalo embryos. Buffalo oocytes were matured and fertilized in vitro by fresh and frozen semen. After embryos evaluation, good quality morula and blastocysts were vitrified using 0.25 ml straws and the post-warmed viability was assessed by further culture for 24 h. There was no significant difference in cleavage rate between embryos derived from fresh and frozen semen, whereas the rate of embryo development to the morula (P

Google ScholarAcdemia.eduResearch GateLinkedinFacebookTwitterGoogle PlusYoutubeWordpressInstagramMendeleyZoteroEvernoteORCIDScopus