The aim of the present study is to evaluate the in vitro maturation rate of camel oocytes using
different maturation media. Ovaries of 50 she-camels were collected during the breeding
season (from December to April). Ovaries were transferred to the lab in a thermal flask
containing warm saline solution within 2 hrs. After slaughtering. Oocytes were collected
using 2 techniques; aspiration of graafian follicles (2-5mm size) and slicing of the ovaries.
Depending on the morphological quality, the collected oocytes were selected and cultured in
different types of maturation media (GTL, HAM’S1, HAM’S 2, HAM’S 3) for 30-40 hours
at 38.5˚c, 5% CO2, and maximum humidity (95-96%). The obtained results revealed that the
slicing technique produced a higher oocyte recovery rate compared to the aspiration
technique. In addition, the maturation media composition mainly the FSH affects the in vitro
maturation rate of camel oocytes. It could be concluded that the type of oocyte maturation
media might be one factor that improve the rate of oocyte maturation.
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