Chlorella vulgaris is one of the better sources of energy. Nitrogen deficiency of Chlorella
vulgaris to the level 0.1mM NaNO3 as shown in results gave the minimum mean growth rate
and highest lipid content (54.88 % oil) as compared with control after 12 days old cultures
(29.7% oil), which represents 84% more than its corresponding control. High salinity to the
level of 0.45 mM also gave the minimum mean growth rate and highest lipid content (43.2 %).
In a similar response to salinity 35.25 μM of ferrous sulphate gave the highest lipid content
(34.7 % oil) as compared with control after 12 days old cultures. Thirteen fatty acids were
identified. Total saturated fatty acids percentages ranged from 19.98-50.34% and total
unsaturated fatty acids ranged from 41.88-80.00%, where the most of the applied treatments
caused an obvious increase in unsaturated fatty acid contents comparing with control, and the
major constituents of the lipid fraction of Chlorella vulgaris was found to be C14, C16, C18,
C18:1, C18:2 and C18:3 fatty acids. Also, it was found that all of the treatments in the present
work resulted in formation of C24 fatty acid which was not recorded in the lipid profile of
untreated alga. |
