This work deals with optimization the production conditions and characterization of cellulases [Carboxymethyl-cellulase (CMCase), Filter-paperase (FPase) and Cellobiase] produced by Gliocladium roseum. Data revealed that Reese and Mandel’s basal medium was the best for maximum protein and cellulases secretion by G. roseum. Maximum protein and cellulases production were found at 15 g/L of rice straw and 2 g/L yeast extract. Maximum yield of protein, CMCase and cellobiase were obtained after 6 days of incubation, while maximum yield of FPase was obtained after 4 days of incubation. After enzymes concentration with ultrafiltration, specific activity of CMCase, FPase and cellobiase were 5.14, 2.5 and 3.9 U/mg protein, respectively. Optimum temperature for CMCase activity was 55 °C, while 50 °C was optimum for the activity of FPase and cellobiase. CMCase retained about 55.63 % of its activity when incubated at 55 °C for 6 h. While, FPase and cellobiase retained about 15.4 and 14.3 % of the original activity when incubated at 50 °C for 6 h, respectively. The optimum pH for CMCase activity was pH 4. While pH 4.5 was optimum for the activity of FPase and cellobiase. An isoelectric point around 5.0 was detected for CMCase. The molecular weight was calculated to be about 50 KDa. |
