Objective: the aim of this st udy is to investigate the in vit ro antioxidant activit y, the total
phenol and flavonoid content and the possible protective effects of commercial propolis on
gentamicin-induced nephrotoxicity in rabbits.
Methods: the in vitro antioxidant activity was measured by ferric reducing antioxidant
power assay and cupric reducing antioxidant capacit y assay, the total phenols content was
measured by folin–ciocalteau assay, the f lavonoids content by the alcl3 colorimet ric method
and the renoprotective effects of propolis methanol extract was evaluated in a rabbit model of
gentamicin-induced nephrotoxicity. The protective effects of propolis on gentamicin-induced
nephrotoxicity in rabbits were evaluated through biochemical parameter (measuring serum
urea and creatinine) and histopathological alterations in kidneys
Results: methanol ext ract of propolis showed a st rong antioxidant activit y, which is
attributed to its high phenolic and flavonoid contents. Oral administration of propolis extract
to rabbits at a dose of 1 mg/kg body weight significantly protected against histopathological
and biochemical alterations induced by gentamicin.
Conclusion: the present study demonstrated that commercial propolis is strong antioxidant
and is effective for the prevention of gentamicin-induced renal damage in rabbits