This study was intended to verify the clinicopathological effect of intra-articular (IA) staph aureus
and amphotericin B injection in the carpal joints of donkeys to induce arthritic changes and to
correlate it with the histopathological findings. Six healthy, adult donkeys of both sexes. Animals
were numbered and divided into two groups; each group consists of three animals. 1st group is used
for induction of septic carpitis in the right radiocarpal joints of donkeys by Staph. aureus with a
dose of 3×106cfu as a single intra-articular injection. The 2nd group is used for induction of
osteoarthritis in the radiocarpal joints of donkeys by 20 mg amphotericin B in 3 ml sterile water as a
single intra-articular injection. Whereas the left joint was the control in both groups .Two ml
Synovial fluid and serum were taken at 0, 1, 3,7,13 days after administration and tissues samples of
Joint capsule and synovial membrane at 13 days. Sampling at zero day was considered as control.
Clinical assessment, synovial fluid analysis, ultrasonography and histopathological examinations
were done. Total leukocytic count, total protein Amyloid A, IL-1β and CRP concentrations sharply
increased on 1, 3, 7, 13days 7 after induction of the experiment when compared with their control at
zero. .There were significant changes in amyloid A, IL-1β and CRP in septic cases when compared
with aseptic cases of arthritis. The results obtained confirm that the concentrations of total
leukocytic count, total protein amyloid A, IL-1β and CRP increase substantially in equine septic
arthritis and osteoarthritis which provides a theoretical basis for the rapid diagnosis of the diseases
and to distinguish between septic arthritis and non-septic synovitis by a variety of diagnostics. |
