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Prof. Alaa El-Sayed Ahmed Ahmed Amin :: Publications:

Utility of tetrazolium blue for the colorimetric assay of tannins in tea.
Authors: Alaa S. Amin.
Year: 1997
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Local/International: Local
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A simple, rapid and accurate colorimetric method is proposed for the determination of tannins, based on the reduction of tetrazolium blue in alkaline medium by tannins at 90 2 C for 15 min, leading to the formation of a highly coloured formazan derivative. Absorbance measurements were made at 527 nm and the calibration graph was linear for 0.2-9.0 g/ml of tannic acid. For more accurate analysis, the Ringbom optimum concentration range was found to be 0.5–8.2 g/ml. The relative standard deviation for the determination in a tea sample containing 7.55% tannins was 1.65%. Most of the ingredients commonly found in tea samples do not interfere with the determination. Several tea samples were analysed using the proposed method.

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