In this work, three different concentrations of Montanide gel adjuvanted RVF vaccine were prepared, the first contains 10%, and the 2nd contains 15% while the 3rd contains 20% Montanide gel, in addition to the aluminium hydroxide gel RVF vaccine to compare. There were no body temperature elevations or any other signs after vaccination in all vaccinated sheep. The humeral immunity in vaccinated sheep was evaluated by using serum neutralization test (SNT) and enzyme linked immunosorbant assay (ELISA), the immunity was detected in groups 2, 3, 4 vaccinated with different concentrations of montanide gel via serum neutralization test from 1st week with values of (1.7, 1.8 and 2) respectively and stayed protective till the 6 months in Group 2, 8 months in Group 3 and 10 months in Group 4 with values of (1.7, 1.8 and 1.8) respectively. These results was correlated to that obtained by ELISA, also the immunity was detected from 1st week in groups 2, 3,4 with values of (0.245, 0.250 and 0.252) respectively and stayed protective till 6th month in Group 2, 8th month in Group 3 and 10th month in Group 4 with values of (0.230, 0.239 and 0.239) respectively. These results revealed that the best vaccine is 20% Montanide gel inactivated RVF vaccine which gave higher level of antibody all over the period of the test compared with that of other vaccinated groups. |
