Tetanus is a fatal infectious disease that affects both human and animals; it was known that the causative organism is Clostridiumtetani which is gram positive, anaerobic bacteria. Tetanus is a wound infection disease that is usually accompanied by a fatal toxemia. Tetanus toxin was inoculated into hens to get hyper immuneIgY, the titer was 1320 Limit of flocculation (Lf-eq) after 72 days and the titration was approved by Ramon flocculation test and single radial immuno-diffusion test. IgY was evaluated experimentally in donkeys as prophylactic and therapeutic medication. IgY could protect donkeys from 1 minimum lethal dose (MLD) ofClostridiumtetanibacteria through passive immunization before and after infection. A dose of 4500 Limit of flocculation (Lf-eq) IgY was 100% protective as a prophylactic dose for a donkey of around100 Kg body weight challenged with 1 minimum lethal dose (MLD) ofClostridiumtetanibacteria. While a dose of 30000 Limit of flocculation (Lf-eq) IgY intramuscularly two times daily for 2 injections, with 9500 Limit of flocculation (Lf-eq) IgYintrathecally (in subarachnoid space through atlanto-occipital space)was 100% curative for a donkey of around100 Kg body weight challenged with 1 minimum lethal dose (MLD) of Clostridiumtetanibacteria. Furthermore, IgY was evaluated experimentally in comparison with IgG in mice. IgY has equally efficacy to IgG in prophylaxis and treatment of tetanus. Thus this trend in tetanus treatment is effective and competitive to IgG treatment. |
