An epidemiological investigation was carried out to evaluate the current situation of Rift Valley Fever disease (RVF) in Egypt. The results of study showed that vaccinated sheep from El-Qalyubia had lower percent of antibodies against RVF with non-protective titer (1/20) while, Vaccinated sheep, goat, cattle and buffaloes from Marsa Matruh and El-Monufia revealed higher percent of antibodies against RVF with protective titer 1/40 using agar gel precipitation test (AGPT), serum neutralization test (SNT) and enzyme linked immunosorbent assay (ELISA). On the other hand non-vaccinated sheep, goats, cattle, buffaloes and camels from El-Qalyubia, El-Dakahlia, El-Sharqia and Kafr El-Sheikh exihibted different rates of antibodies against RVF among governorates in which higher rate occur in El-Sharqia followed by El-Dakahlia then Kafr El-Sheikh and lastly Qualubya. The virological analysis showed no virus isolate from sera samples of all governorates or from liver suspension of rats collected from El-Qalyubia, El-Dakahlia, El-Sharqia and Kafr el-Sheikh. Results of PCR showed that RNA of RVFV was not detected in sera samples and the cell culture in the examined animals neither from El-Sharqia and Kafr El-Sheikh governorates nor from liver suspension of rats collected from El-Qalyubia, El-Dakahlia, El-Sharqia and Kafr El-Sheikh using RT-PCR and real time PCR. On conclusion there is no circulating virus among the examined governorate under study. |
