A total of 405 cattle of different ages, sexes, and breeds were examined clinically for diagnosis of babesiosis and theileriosis. Blood film examination revealed that 98 out of 405 animals showed either Babesia organism or the intraerthrocytic stage of Theileria inside the red blood cells (RBCs). 33 (8.1%) smear and 65 (16.05%) were positive for Babesia and Theileria, respectively. By examination of 158 serum samples by IFAT for detecting the presence of antibodies against either Babesia or Theileria species, 25 samples (15.8%) were positive for Babesia antibodies and 33 serum samples (20.9%) were positive for Theileria antibodies. For confirming all above methods of diagnosis, we had been used PCR in which 275 blood samples were examined after DNA extraction for the presence of either Babesia or Theileria. The results revealed that positive samples for Babesia were 24 sample (8.73%) and 60 sample (21.82%) were positive for Theileria. By comparing the different methods of diagnosis, we found that out of 158 cattle examined by three methods 12 samples were positive for blood parasites by three methods of diagnosis (Direct smear, IFAT, PCR), 45 samples were positive by PCR and direct smear, 4 samples positive by PCR and IFAT, 4 sample was positive by PCR only, and 41 samples positive by IFAT only |
