With the aim of preparation of diagnostic antigens for Candida albicans, three types
of Candida subcellular components were extracted by mechanical and enzymatic
extractions. Different methods of extraction frequently used in other studies were used
to release proteins form intact cells of Candida albicans. Extract were obtained from
intact cells by (i) sonication, (ii) treatment with Zymolyase and Trichoderma lysing
enzymes (TLE), and (iii) sonication after enzymatic treatment. The extracts were
separated with SDS- polyacrylamide gel electrophoresis. The antigenicity and the cross
reactivity of these fractions were analyzed by using the ELISA technique. The results
revealed that the three fractions are antigenic. The whole cell and the cell wall fractions
were more antigenic than the membrane fraction. There were various degrees of cross
reactivity between these fractions. Moreover, the specificity of these antigens was
examined. The results revealed that these antigens were strict specific to C. albicans
only. The results presented in this study could be considered as a step for development
of diagnostic and immunotherapeutic products for detection and controlling the
Candida albicans infections among animals. |
