For the present study the harmful effects of organophosphorous pesticides chronic toxicity on several
biochemical parameters and evaluation of the possible protective effects of chlorophyllin as
antioxidant in rats exposed to Pirimiphos methyl (POM), one of organophosphorous pesticides widely
used in the middle east, were investigated. Sixty adult white male albino rats weighting 150 - 200 gm
were used in this study. The rats were divided into four equal groups. 1) Normal group (N): received
no drugs. 2) POM group (P): received single dose of POM (50 mg/ Kg b.w) daily for 3 months. 3)
Chlorophyllin group (Chl): received single oral dose of Chlorophyllin (27 mg/ Kg b.w) daily for 3
months. 4) Chlorophyllin + POM group (Chl + P): received single dose of POM (50 mg/ Kg b.w) +
Chlorophyllin (27 mg/ Kg b.w) per os daily for 3 months. Blood and brain samples were collected
from all animal groups three times at one, two and three months from the onset of experiment.
Plasma was separated for determination of Ceruloplasmin, NO and γGT. Moreover, after plasma
separation, erythrocytes were washed for determination of AChE, GSH, MDA, CAT, SOD, GPx, GR
and GST. Brain AChE, GSH, MDA, CAT, were also determined. The obtained results revealed that,
administration of Pirimiphos methyl exhibited a significant decrease in erythrocytes and brain AChE,
CAT, GSH, erythrocytes GPx, GR and GST activitie,. CHL administration in POM intoxicated rats
exhibited significant increase in all mentioned parameters. On the other hand, a marked increase in
erythrocytes SOD, serum GGT activities, erythrocytes and brain MDA and serum NO level were
observed. CHL administration in POM intoxicated rats exhibited significant decrease in all mentioned
parameters. From the obtained results, it could be concluded that, the potential of CHL as natural
antioxidant act as a powerful agent against the toxic effects of POM. |
