Background: One-third of the world’s population is infected with Mycobacterium tuberculosis. Difference in clinical outcome of infection implies that host genetics may be implicated in such variability. Investigations of Toll-like receptors (TLRs) revealed new information regarding the immunopathogenesis of tuberculosis. Toll-like receptor 2 (TLR2) mediates crucial immune response against Mycobacterium tuberculosis. There is argument that Toll-like receptor (TLR10) participate in tuberculosis susceptibility by acting as a signaling modulator for TLR2. Objectives: The aim of this study was investigating the relationship between TLR 10 SNP 720A/C (rs11096957) and increase susceptibility to tuberculosis. Methodology: Eighty patients with radiological, microbiological and clinical proven active pulmonary tuberculosis (T.B) were included in this study. (TLR10) polymorphisms and allele distributions were compared between these 80 patients and 70 healthy control subjects. Peripheral blood samples were taken from all patients and controls. Genotyping was accomplished by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: When we compare T.B cases with controls, a statistically significant association was observed between T.B susceptibility and SNP 720A/C (rs11096957) in (TLR10). Allele (A) was more frequent in tuberculous cases while allele (C) was more common in controls. It was reported that the AA genotype of (TLR10) SNP rs11096957 was considerably related to the increased risk of developing pulmonary T.B. Homozygosity (AA) has been associated with predisposition to disease by comparing cases to controls (P = 0.045; OR = 2.0; 95% C.I. = 1.0- 4.0). A/C heterozygosity was considerably different in tuberculous cases than in healthy controls with lower risk of developing tuberculosis (P = 0.044; OR = 0.5; 95% C.I. = 0.26 –0.98). Conclusion: TLR10 SNP rs11096957 polymorphism is a risk factor for tuberculosis infection. |
