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Dr. Al Shimaa Al Hussainy Hasab Elnabi :: Publications:

Influence of Epidermal Growth Factor with Cysteamine on in -Vitro Buffalo Embryo Development
Authors: 1Al-shimaa Al-H. H. El-Naby, 2Karima Gh.M. Mahmoud,3Scholkamy, T.H. 2Youssef F. Ahmed, 1Gamal A.M. Sosa and1Mahmoud E.A. Abouel-Roos
Year: 2016
Keywords: Buffalo oocytes, EGF, cysteamine, embryo development, culture media.
Journal: Egyptian Journal of Veterinary Sciences
Volume: accepted
Issue: Not Available
Pages: Not Available
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper Not Available
Supplementary materials Not Available

Growth factors supplementation had a mitogenic effect in numerous species. Two experiments were conducted for improving embryo development in buffalo by addition of epidermal growth factors and cysteamine as antioxidant. The first one was carried out to evaluate the different concentrations (0, 5, 25, 50 ng/ml) of epidermal growth factors (EGF) on developmental competence of buffalo oocytes. Buffalo oocytes were cultured in the four concentrations of EGF. The embryo cleavage rate was significantly higher in oocytes exposed to 5, 25, 50 ng/ml EGF than control. There were no significant difference in four groups (0, 5, 25, 50 ng/ml EGF) in the rate of morula. But, better cleavage and blastocyst rates were observed at 5 ng/ml EG. In the second experiment, the additive effect of 5 ng/ml EGF with 50 µM cysteamine on maturation and embryo development was studied. Oocytes were collected, matured and cultured in media supplemented with 5 ng/ml EGF and 50 µM cysteamine combination (first group), single supplementation with EGF (second group) and cysteamine alone(third group). There was a significant increase in cleavage rate in combination group than EGF (P< 0.05) and cysteamine (P< 0.01) groups. But there was no significant difference in cleavage rate between EGF and cysteamine. The morula percentage was nearly similar in the three groups. but blastocyst rate was significantly (P

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