This work was carried out on 30 Fasciola positive patients of both sexes, aged from 5-56 years old, 20 patients infected with other parasites than Fasciola and 20 healthy individu als (normal control). Detection of anti Fasciola IgG, 1gM, IgA and IgE antibodies was done by indirect ELISA assay. Anti-Fasciola IgG antibodies were detected in 93% of cases with sensitivity 93% and specificity 80%, 1gM antibodies were detected in 83% of cases with sensitivity 83% and specificity 90%, IgE antibodies were detected in 33% of cases with sensitiv ity 33% and specificity 100% and IgA antibodies were detected in 70% of cases with sensitivity 70% and speci ficity 72.5%. By using competitive inhibition ELISA technique, Fasciola excretory / secretory (E/S) antigen was detected in 20% of cases with sensitiv ity 20% and specificity 92.5%. Both of anti-Fascio!a IgG and 1gM antibodies were detected in all Fasciola antigen positive cases. The highest specificity for Fasciola
diagnosis assays was that of IgE, the lowest was that of IgA ELISA assay. It was concluded that the highest sensitivity for Fasciola di agnosis was that of anti-Fasciola IgG ELISA and the lowest was that of competitive inhibition ELISA assay for detection of Fasciola antigen
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