E-learning is the use of internet technology to enable students to learn anytime and anywhere. It can include training, the delivery of just-in-time information and guidance from experts. Hence, it has an increasingly popular learning approach in higher educational institutions-learning must be a part of strategy in many institutions trying to make success in basic education, higher education, continuing education, and training. Design: An intervention (Quasi experimental) study design was used. Aim: To study effect of E-learning on knowledge retention and student's achievement in obstetrical and gynecological nursing curriculum. Setting: The study was conducted at Faculty of Nursing Benha University. (Obstetrical and Gynecological Department). Subjects and methods: A purposive samplewithan intervention group of 80 third year students enrolled for the course of obstetrical and gynecological nursing using e-learning and 78 studentstaught by the traditional method as control. Tools: 1) Self-administered questionnaireto assess: - Student’s overallsatisfaction with the traditional method of teaching the curriculum for the control group. Student’s satisfaction as well as the opinions regarding advantages and barriers of E-learning for the intervention group. 2) Knowledge assessment sheet to assess student's knowledgeacquisition and retention as well as: A pre and posttest exam of each unit for the students & Final academic score result to assess student's final grads in obstetrical and gynecological nursing curriculum. Results: All students in both groups had low scores in the pretest. At the posttest and final exams the scores were statistically significantly higher among students in the intervention group in compared with those in the control group (p |