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Dr. Amal Mohammed Mohammed Saeed :: Publications:

Nested Polymerase Chain Reaction versus Enzyme Linked Immunosorbent Assay for Rapid Diagnosis of Sinus Aspergillosis
Authors: 1Amal M. Saeed*, 1Nehad A. Fouad, 2Mohamed Elshaboury
Year: 2019
Keywords: Rhinonasal aspergillosis, Nested PCR, ELISA, Galactomannan
Journal: Not Available
Volume: Not Available
Issue: Not Available
Pages: Not Available
Publisher: Not Available
Local/International: Local
Paper Link: Not Available
Full paper Amal Mohammed Mohammed Saeed_7.pdf
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Background: Fungal infections of the nose and paranasal sinuses are classified into invasive and non invasive types. Rapid detection and identification of causative fungi is crucial for effective treatment and avoidance of complications. Objectives: To evaluate diagnostic outcome of ELISA detection of galactomannan (GM) in sinus lavage aspirate as a rapid minimally invasive diagnostic test for Aspergillus spp. sinus infection in comparison to fungus DNA detection by nested PCR. Methodology: 95 chronic rhinosinusitis (CRS) patients had manifestations since ≥12 weeks despite of medical therapy were clinically and radiologically evaluated and underwent sinus lavage. Lavage aspirate was used for microbiological examination, PCR testing for Aspergillus DNA and ELISA detection of GM. Results: PCR defined Aspergillus DNA in 56 samples, culture defined 53 positive samples for Aspergillus spp. infection and ELISA detected GM antigen in 49 samples. In comparison to PCR result, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) and accuracy rates of culture were 73.2%, 69.3%, 77.4%, 64.3% and 71.6% while ELISA detected GM antigen were 84.6%, 88.4%, 89.8%, 82.6% and 86.3%, respectively. ELISA results significantly correlated with results of culture and PCR with a positive significant correlation between PCR and culture results. ROC curve analysis defined ELISA detection of GM as more significant predictor for result of culture (AUC =0.792, p

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