Background: Sepsis is a life-threatening organ dysfunction caused by a dysregulated
host response to infection. Soluble Programmed cell death protein-1(sPD-1) increased in
patients with sepsis and involved in immunosuppression. It is associated with a poor
prognosis, severity and 28-day mortality. Objectives: Identification of the caustive
agents of sepsis using Bact Alert 3D system, measurement of serum level of sPD-1 in
patients with sepsis compared to control group by ELISA and evaluation of sPD-1 as a
diagnostic and prognostic marker for the severity and 28-day mortality. Methodology:
Our study was done on 40 patients diagnosed as sepsis in Chest and Intensive Care
Units Departments of Benha University Hospital. Blood samples were withdrawn from
the patients for blood culture, Subcultures and biochemical tests were done for
identification of the caustive agents of sepsis and ELISA is used for for measurment of
sPD-1 level in patients and control group. Results: According to the causative
pathogens, Klebsiella pneumoniae was the most frequently isolated organism (32.5%),
followed by Pseudomonas aeruginosa (22.5%) and Staphylococcus aureus (17.5%).
According to sPD-1, the mean serum level was significantly higher in the case group
(17.58 ± 15.05 pg/mL) compared to the control group (0.53 ± 0.37 pg/mL). SPD-1 levels
were markedly higher in non-survivors compared to survivors. Conclusion: sepsis is a
common disease with multimicrobial causes. Klebsiella pneumoniae is the main
causative bacteria for sepsis. sPD-1 is a promising diagnostic marker for sepsis with
high sensitivity (97.5%), and specificity (95.0%), and prognostic marker for the severity
and 28-day mortality |
