Nanoparticles (NPs) are now extensively used in different fields with inadequate knowledge on their
toxicity. Salmeterol is a potent long acting-β agonist (LABA) used for the management of pulmonary
disorders. Sothe present study was aimed to evaluating in vitro cytotoxicity and genotoxicity of nano
salmeterol xinafoate nebulization. Blood samples from ten healthy volunteers were cultured and divided
into two equal groups: control untreated group and other treated with a dose of nano salmeterol (50μg/ml)
and incubated at 37°C for 24 hour then evaluated for local and systemic toxicity. Genotoxic study by
using Comet assay and chromosomal karyotype was done. Morphological analysis of nano-sized
salmeterol xinafoate particles was performed by Transmission Electron Microscopy (TEM) and Anderson
Cascade Impactor (ACI) in which the average particle size was in the range of 300–500 nm. Our results
showed that there was a- non significant decrease (p>0.0.05) in the level of antioxidant enzymes: ROS,
GSH, SOD, and Catalase; while, there was significant increase (p0.0.05), Also, the
chromosomal aberration was non-significant increase (p>0.0.05). So our study concluded that this dose
of nano-salmeterol xinafoate NP nebulizer may be tolerated by asthmatic patients and further studies
need in diseased patients and with different organs. |
