Background: Amiodarone is an antiarrhythmic drug. It causes
thyroid dysfunction. Objective: To explore the possible
therapeutic effect of L-carnitine and exosomes on amiodarone
induced thyroid follicular cells toxicity. Materials and Methods:
Sixty-two rats divided into 7 groups. Group I (control), group II
(they were treated with amiodarone orally in a dose of 60 mg/kg
for 6 weeks, group III (they were treated with L-carnitine in a
dose of 500 mg/ kg for 4weeks),and group IV (they were treated
with exosomes which were given in 3 doses of exosomes. Some
rats from this group were sacrificed immediately after the last
dose. While, The remaining rats stayed another 10 days formed
group VII, group V (they were treated with L-carnitine and
exosomes together), group VI (recovery group) and Group VII
(exosomes withdrawal). Results: Groups II and VI showed loss
of normal follicular architecture. The follicular epithelial cells
showed more than one layer with small dark nuclei. The
interfollicular tissue showed congested vessels and infiltrates.
Ultrathin sections of these groups showed follicular epithelium
with irregular nuclei. The cytoplasm showed dilated rough
endoplasmic reticulum and degenerated mitochondria.
Furthermore, there was intense positive collagen and Ki-67 in
these groups (p< 0.01). Groups III, IV, V and VII showed
improvement. Biochemical Results: a significant increase in TSH
in groups II and VI in comparison to control. While, there was a
significant decrease in the levels of FT3 and FT4. Serum TSH
levels were decreased in groups III, IV, VII and V. |
