| Abstract |
The aim of the present study was to investigate the effect of plant growth regulators (TDZ and BAP) and silver nanoparticles Ag-NPs on in vitro microtuberization in potato cv. Spunta. Different concentrations (4, 6, 8 and 10 mg/l) of BAP either alone or in combination with four concentrations (0,1, 2 and 3 mg/l) of TDZ were used for investigating their influence on microtuberization. The obtained results showed that, the individual treatment of BAP at the high level (10 mg/l) recorded the highest average number of the produced microtubers/jar (7.1) compared to the other treatments (4, 6 and 8 mg/l). The combinational treatments of BAP and TDZ exhibited an increase compared to the individual corresponding concentration of BAP and this increase reached the significant level with the concentrations (4, 6 and 8 mg/l) of BAP, while a slight increase was recorded with 10 mg/l. Furthermore, the combinational treatment of BAP (6 mg/l) and TDZ (1 mg/l) resulted in the highest average number of the produced microtubers/jar (8.1) which represents about 41.1 % increase over the individual treatment of BAP (6 mg/l), the highest average weight of microtuber (116.94 mg) and the highest average diameter of microtuber (5.3 mm). These results confirm the positive effect of the combinational treatment of BAP and TDZ on enhancing microtuber formation in potato cv. Spunta. Accordingly, this treatment of BAP (6 mg/l) and TDZ (1 mg/l) was used in the experiment of Ag-NPs and silver thiosulphate (STS). The results of Ag-NPs treatments showed that, the number of microtubers/jar was elevated and reached highest value (14.9 microtubers/jar) which represents about 83.95 % over the control treatment (8.1 microtubers/jar) using the medium containing (Ag-NPs 1 mg/l). While (10.9 microtubers/jar) was the highest value recorded using the medium containing (STS 8 mg/l) which represents about 34.56 % over the control treatment. Thus, these results clearly confirmed that the low level of Ag-NPs (1mg/l) was the most effective concentration for increasing microtubers production in potato cv. Spunta. Therefore, we report here that an efficient protocol for microtuberization in potato cv. Spunta using Ag-NPs was established. In addition, these promising results are important for developing an advanced technology for microtubers mass production in Egypt. |
