The present study was performed on 150 random samples of local frozen and imported frozen chicken meat (75 for each), collected from different shops at Kaliobia Governorate to throw light over the bacterial status of them beside the phenotypic characterization of the isolated bacterial strains and detection of some virulence genes in some strains. The bacteriological examination of samples cleared that, a total of 93(62.0%) isolates of foodborne pathogens were recovered from 150 samples (37 from local frozen chicken meat and 56 from imported frozen chicken meat) where S. aureus were the most isolated (31=20.7%, 17 from imported frozen samples and 14 from local frozen ones ) followed by Ps. aeruginosa (18=12.0%, 10 from imported and 8 from local ones); E.coli (11=7.3%,7 from imported and 4 from local ones); Enterobacter diversus (8=5.3%, 5 from imported and 3 from local ones); A. hydrophila and Kl. pneumoniae (7=4.7% , 5 from imported and 2 from local ones for each); Micrococcus spp. (6=4.0%, 4 from imported and 2 from local ones) and Proteus vulgaris (5=3.3%,3 from imported frozen samples and 2 from local frozen ones) .Meanwhile, Salmonellae failed to be isolated from all samples. In addition, the results of antibiotic sensitivity tests for the isolated strains appeared that: For E.coli, they were highly resistant for amoxicillin; ampicillin and oxytetracycline followed by methicillin but they were highly sensitive to enrofloxacin and gentamycin followed by norfloxacin, cefotaxime and ciprofloxacin. For S.aureus, they were highly resistant for oxacillin followed by methicillin; nalidixic acid; ampicillin; amoxicillin and oxytetracycline but they were highly sensitive to gentamycin followed by enrofloxacin and norfloxacin. For Ps. aeruginosa, they were highly resistant for oxacillin followed by amoxicillin, ampicillin and methicillin but they were highly sensitive to enrofloxacin and norfloxacin followed by gentamycin and ciprofloxacin. In addition, for A. hydrophila, they were highly resistant for methicillin and oxacillin followed by amoxicillin, ampicillin, erythromycin, oxytetracycline and streptomycin. Meanwhile, they were highly sensitive to ciprofloxacin and enrofloxacin followed by norfloxacin and gentamycin. PCR results cleared that eaeA and blaSHV virulence genes were detected in the two studied E.coli strains; clfA and mecA virulence genes were detected in the two studied S.aureus strains and regarding to A. hydrophila strains; the haemolysin virulence gene was detected in one strain, while bla TEM virulence gene was detected in the two studied strains. |
