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Dr. Assistant prof. Ashraf Mohamed Abd Ellatief Nasif :: Publications: |
|
| Title: | Saprolegniosis in goldfish
(
Carassius auratus
) associate
d
with
novel strain; molecular characterization and electron scanning |
| Authors: | ShaheenA A 1, AM El Asely*1, A M Abd EL Latif1,MM A Moustafa2and H E Elsaied |
| Year: | 2015 |
| Keywords: | Saprolegnia parasitica new strain, Carassius auratus , molecular characterization, Electron microscope |
| Journal: | E gy. J . Aquac |
| Volume: | 5 |
| Issue: | 2090 - 7877 |
| Pages: | 1-12 |
| Publisher: | Not Available |
| Local/International: | International |
| Paper Link: | Not Available |
| Full paper | Ashraf Mohamed Abd Ellatief Nasif _saprolegnisis.pdf |
| Supplementary materials | Not Available |
| Abstract: |
A novel Saprolegnia parasitica strain was isolated from moribund and freshly dead g oldfish , Carassius auratus , fingerlings during mid February 2014 . Fish showed extensive hyphal growth on the skin, fins, gills and eyes as if it is warped with cotton. The isolate was identified using the traditional methods through its asexual and sexual organs, followed by 18 S rRNA gene sequenc ing ; showing identity 91.6% with S. parasitica . Scanning electron microscopy was applied for evaluating the pathogenicity of the retrieved isolate; which proved its high virulence accompanied with the high mortality rate reaching 100% . Keywords: Saprolegnia parasitica new strain, Carassius auratus , molecular characterization, Electron microscope. I |
