Silver nanoparticles (AgNP) are one of the most synthesised nanomaterials due to their widespread applications,
which in turn increases the potential of release and accumulation in nature. Moreover, they are known as
antibacterial and recommended to be used in different commercial drugs. However, the evaluation of their effect
on living organisms, especially fish, at low levels is still not completely understood. Therefore, the present study
was conducted to investigate the effects of aqueous exposure of AgNPs on growth performance, oxidative status,
non-specific immunity, and histopathological changes of epidermis, gills, liver, and intestine of Nile Tilapia,
Oreochromis niloticus. The fish were exposed to four concentrations of AgNP (0, 10, 20, and 30 μg L-1) for 8 weeks.
At the end of the experiment, fish were intraperitoneally injected with pathogenic bacteria Aeromonas hydrophila
and fish mortalities were recorded up to 14 days post-challenge. The results revealed that growth performance
and feed utilisation of fish exposed to the lower AgNPs concentration improved significantly compared to other
treatments. The increasing AgNP exposure levels decreased different growth indicators in a dose-dependent
manner. The highest catalase, superoxide dismutase, glutathione peroxidase, lysozyme, and respiratory burst
activities were recorded with 10 μg AgNPs L− 1 and the enzyme activities were retarded with increasing AgNP
concentrations. The total bacteria count in the water decreased with increasing AgNP concentrations. Meanwhile, the relative percentage of survival improved with low AgNP concentration and decreased with increasing
exposure levels. The histopathological investigation of different studied tissues (gills, skin, liver, and intestine)
revealed an increase of histological lesions’ frequency and severity with increasing AgNP concentrations. It can
be concluded that the exposure of Nile Tilapia to AgNP at levels exceeding 10 μg L− 1 could interrupt growth
performance, immunity, and antioxidant status and induced histopathological changes. |
