Diagnosis of the BVDV infection among suspected and apparently healthy cattle at Kaluobia, Giza, Menofeia and Gharbia governorates was done by detection of prevalence of BVD antibodies. A total number of 151/151(100%) and 97/151 (62.25%) of examined sera were positive for BVD antibodies using serum neutralization test (SNT) and competitive immunoenzymatic assay (cIEA), respectively. Examined sera with cIEA detected antibodies against BVDV non structral proteins P80/P125. Detection of BVDV in buffy coat samples using antigen capture ELISA showed that 22/151(14.56%) of the samples were positive for BVDV. Isolation and biotyping of suspected BVDV from buffy coat on MDBK cell line showed that 19/22 of ELISA positive buffy coat samples were cytopathogenic BVDV biotype (cpBVDV) while only 3/22 samples were CPE negative suggesting a noncytopathogenic BVDV (ncpBVDV) biotype. Inoculated cell culture with no CPE were subjected to IFAT and IPMA using specific antisera against BVDV revealed positive results indicating presence of non-cytopathogenic strain of BVDV. It was concluded that cIEA detected antibodies against nonstructural proteins P80/P125 has many advantages over SNT being for rapid diagnosis of BVDV. However, diagnosis must be confirmed with isolation, biotyping and identification of BVDV using suitable sensitive and specific methods as ELISA, IFAT and IPMA. |
