You are in:Home/Publications/Isolation And Molecular Identification Of Bovine Herpes Virus-1 Among Infected Buffaloes From Kalubia Governorate, Egypt | |
Prof. Ayman Said Emam Desoky El-Habbaa :: Publications: |
Title: | Isolation And Molecular Identification Of Bovine Herpes Virus-1 Among Infected Buffaloes From Kalubia Governorate, Egypt |
Authors: | AS El-Habbaa |
Year: | 2012 |
Keywords: | Bovine Herpes virus -1,Buffaloes, Kalubia, Egypt, |
Journal: | Egyptian Journal Of Virology |
Volume: | 9 |
Issue: | 2 |
Pages: | 465 – 476 |
Publisher: | Egyptian Syndicate of Virology |
Local/International: | Local |
Paper Link: | Not Available |
Full paper | Not Available |
Supplementary materials | Not Available |
Abstract: |
One hundred samples from clinically suspected buffaloes for Bovine Herpes virus -1 infection at Kalubia governorate were subjected for trials of virus isolation on MDBK cell line. Nine positive samples as follows 1 out of 25, 6 out of 25, 1 out of 25 and 1 out of 25 of ocular, nasal, vaginal swabs and tissue samples, respectively. Positive samples upon isolation were identified with indirect fluorescent antibody technique (IFAT) and revealed that 2, 1, and 1 were positive for nasal swabs, vaginal swabs and tissue samples respectively. Polymerase chain reaction (PCR) for glycoprotein B (gB) gene have also been used for molecular identification. |