| Abstract |
Heavy metals stress is one of the major abiotic anxieties that cause
environmental pollution and hence open-ended hazards to living organisms in recent
eras. The current study was established with the goal in the mind to clarify the
ameliorative role of seed presoaking in kinetin (30 ppm), calcium chloride (40 mm)
and salicylic acid (0.1 mM) either separately or in alternate combination in offsetting
the deteriorative effect of foliar application of nickel (0.5 mM) and/or lead (2.5mM) on
the growth parameters and some metabolic activities of Phaseolus vulgaris L. plant
variety Giza 6. Nickel and/or lead foliar application non-significantly reduced the
shoot length, leaves number ,leaves area, fresh and dry weights of shoot and
significantly reduced the membrane stability index, total pigments, ,soluble and
insoluble sugars, total protein, total nitrogen, carbonic anhydrase, nitrate reductase,
catalase, , potassium, calcium and magnesium contents. On the other hand,
electrolyte leakage, proline, amino-N, SOD, POX, MDA, total phenols, nickel and
lead contents were significantly increased as a comeback for heavy metal
treatments. On contrary, seed presoaking in kinetin, calcium chloride and salicylic
acid either separately or in alternate combination non-significantly increased the
shoot length, leaves number, leaves area fresh and dry weights of shoot and
significantly increased the membrane stability index, total pigments, soluble and
insoluble sugars, amino nitrogen, total protein, total nitrogen, carbonic anhydrase,
nitrate reductase, catalase, potassium, calcium and magnesium contents. Whereas,
electrolyte leakage, proline, SOD, POX, MDA, total phenols, nickel and lead contents
were significantly decreased by the same presoaking treatments. Kinetin, calcium
chloride and/or salicylic acid combined treatments were found to be more effective in
enhancing the plant tolerance towards nickel and/or lead venomousness. Also,
nickel was found to be more injurious to bean growth than lead.
The different presoaking and metal treatments markedly altered the protein
profile of P. vulgaris via inducing the de novo synthesis of some protein bands and
causing the disappearance of other bands |
