The objectives of this study were to detect genetic polymorphism in exon 10 of Follicle
Stimulating Hormone Receptor gene (FSHR) by using Restriction Fragment Length
Polymorphism (RFLP) and direct sequence methods, also to investigate their possible
association with infertility traits in Egyptian buffaloes. A total number of 50 female Egyptian
buffaloes at Mahallet Mousa experimental farms in Kafr El-sheikh province were
investigated. Animals were divided into three groups: normal fertile (20 animals), infertile
due to Anestrum (15 animals) and infertile due to repeat breeding (15 animals). Genomic
DNA was extracted from blood samples, then PCR was performed with annealing
temperatures of 57 ºC to amplify 231 bp of FSHR exon 10 gene. The amplicons were
digested with Hin1II (NlaIII) restriction enzyme. The results showed that all the investigated
buffaloes were monomorphic and genotyped as A/A genotype. Moreover, the monomorphism
of buffaloes FSHR exon 10 gene was confirmed by sequencing three samples from each
buffaloes’ group. It seems that FSHR gene are highly conserved in the investigated buffalo’s
population. |
