Background: Diabetes mellitus is a major health problem in the world which characterized by multiple metabolic diseases such as hyperglycemia, obesity, dyslipidemia and hypertension. Type 2 diabetes (T2D) is the commonest type of diabetes and rang from predominantly insulin resistance and relative insulin secretion. Protein tyrosine phosphatase 1B (PTPN1) plays an important role in insulin signaling pathways. It is dephosphorylates insulin receptor and insulin receptor substrates so PTP1B acts as insulin negative regulator and may be an effective target for the treatment of type 2 diabetes. This study aimed to detect the association of 467T>C and 1023C>A gene polymorphisms with insulin resistance in Type 2 diabetes in Egyptian patients. Method: Detection of 467T>C and 1023C>A gene polymorphisms in Type 2 diabetic patients were processed by Polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP). 35 Egyptian patients with T2D and 15 controls enrolled in the present study. Results: our study did not observe any significant difference in 467T>C PTPN1 genotypes between patients' group and control group (FET=2.21, P=0.35). The C allele was more frequent allele in diabetic patients' group (81.4%) and control group (70%) but without significant association in the studied groups (P>0.05). Also, the 1023C>A PTPN1genotype was not significantly associated with T2D (FET=0.87, P=0.79). The C allele was more frequent allele in patients' group (92.9%) and control group (90%) but without significant association in the studied groups (P>0.05). 1023C>A and 467T>C PTPN1 variants showed non-significant association with metabolic disorders in studied groups but 1023C>A PTPN1genotype was significantly associated with hypertensive T2D (P=0.006, FET=8.55). Conclusion: The PTPN1 promoter variants 1023C>A and 467T>C were not associated with insulin resistance in Type 2 diabetes in Egyptian patients and metabolic traits in this study but we found significant association between hypertensiveT2D and 1023C>A PTPN1 polymorphism. |
