Background: Different types of miRNA were discovered to have a big role now in many diseases and cancer.
Objectives: This study assessed the association between the miRNA-196a2 (rs 11614913) polymorphism and acute
lymphoblastic leukemia (ALL) in children.
Design: Retrospective case-control study.
Settings: This study was done in Benha University Hospital Pediatric Department & Benha Specialist Hospital for
Children from May 2019 to February 2020.
Subjects and methods: Blood DNA samples from cases and control children were studied for the miRNA-196a2
(rs11614913) polymorphism using Polymerase Chain Reaction-Restriction Fragment-Length Polymorphism
(PCR-RFLP) approach.
Sample size: 100 childhood ALL patients and 60 apparently healthy children as control were recruited in our
study.
Results: The frequency of miRNA-196a2 (rs 11614913) C allele was 56.7% in control compared to 54% in cases.
In addition there was no significant association between CC or TT genotypes and susceptibility of pediatric acute
lymphoblastic leukemia (P = 0.74 and 0.36). There was statistically highly significant increase in TT genotype in
males compared to females and significant increase in mean of LDH and ESR in TT genotype compared to CC
genotype.
Conclusion: We concluded there was no association between miRNA-196a2 genotypes and pediatric acute
lymphoblastic leukemia in Egypt.
Limitations: Small patient sample size. |
