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Molecular therapeutic role microRNA 221 inhibitor in hepatocellular carcinoma
Authors: Amal Abou El-fadle Hassan 1 , Naglaa Ibrahim Azab 2, Sania khairy Elwia 3, Doaa Shaban Mahmoud 4
Year: 2019
Keywords: Not Available
Journal: Not Available
Volume: Not Available
Issue: Not Available
Pages: Not Available
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper doaa shaban mahmoud mohamed_HCC.docx
Supplementary materials Not Available

Background MicroRNAs play important role in post-transcriptional regulation of gene expression also in development and cellular processes., miR-221 is an oncogenic miRNA that plays important role in the initiation and progression of HCC, by affecting pro-oncogenic pathways thus miRNAs is a new class of targets for the development of miRNA-based therapeutic strategy. The mature miRNA act by competion with cellular target mRNAs moreover that leads to inhibition of the mRNA and expression of its direct targets Aim of the work The aim of this study is to investigate the effect of miRNA221 inhibitor on suppression of proliferation, induction of apoptosis in HCC cell line that may be a novel approach to be a therapy for HCC Material & methods HepG2 HCC cell line MiR-221 inhibitor will be transfected in HCC cell line using ultrasound microbubbles After transfection; Reverse transcription polymerase chain reaction (RT-QPCR) of CDKN1B/P27 genes and miR 221, MTT assay, flow cytometry for the effect of MiR-221 inhibitor on cell growth and apoptosis, caspase-3 activity measurment will be done Results Our data revealed that the delivery of anti-miR-221 by ultrasound microbubble contrast agents. In this study, cell proliferation inhibited and promoted the apoptosis of cells the expression of miR-221 decreased. Consequently, there was upregulation in the expression of CDKN1B/p27 Conclusion The transfection of recombinant plasmids contained anti-miR-221 to suppress oncogenic effect of-miR-221 and cell proliferation was inhibited and cell apoptosis was induced in HepG2 cells. This indicated that effective role miRNA that may be novel gene therapy targets

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