Inactivated tissue culture adapted Rift Valley Fever (RVF) Virus vaccines were prepared using peanut
oil; code liver oil, Montanide ISA 206 oil and aluminum hydroxide gel as adjuvants. The prepared
vaccines were sterile and safe inducing no systemic or local clinical signs in lambs. Comparative
evaluation of four experimentally prepared vaccines in sheep after a single dose indicated that the oily
prepared vaccines greatly stimulated the humoral immune response as estimated by SNT and ELISA
compared with aluminum hydroxide gel vaccine. Protective serum antibody titers against peanut oil and
Montanide ISA 206 oil adjuvanted RVF virus vaccine started at 2nd week post vaccination (WPV)
while code liver oil and Aluminum hydroxide gel vaccine started at 3rd WPV. These protective titers
persisted till the 44th WPV for Montanide ISA 206 oil, 32th WPV for peanut oil and code liver oil and
24th WPV for Aluminum hydroxide gel vaccines then declined under the protective level. Both of SNT
and ELISA indicated that among the used adjuvants, montanide ISA 206 was preferable as they could
be used for manual preparation of the vaccines furthermore the superior immunological response
followed by peanut oil then code liver oil and the aluminum hydroxide gel. |
