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Prof. Ehab Mostafa El-Nahas Mohammed Nassar :: Publications:

Title:
Frequency of rotavirus detection by a sandwich ELISA in feces of diarrheic bovine calves from Qalubia province, Egypt
Authors: Gabr F. El-Bagoury , Ehab M. El-Nahas, Suzan S Sharaf El-Deen, Sayed A.H. Salem
Year: 2014
Keywords: Rotavirus, Sandwich ELISA, Bovine calves, Seasonal pattern 
Journal: BENHA VETERINARY MEDICAL JOURNAL
Volume: 27
Issue: 2
Pages: 341-347
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper Ehab Mostafa El-Nahas Mohammed Nassar _Rotavirus 2014.PDF
Supplementary materials Not Available
Abstract:

This work aimed to study the prevalence of rotavirus in diarrheic feces from bovine calves and the sensitive’s parameters such as age group and seasonal pattern. A total of 250 fecal samples were collected from diarrheic bovine calves in different localities at Qaluobia province, Egypt all over the four seasons of the years 2013 and 2014. These samples were tested for rotavirus antigen by sandwich Enzyme linked immunosorbent assay (ELISA). Rotavirus antigen was detected in 30% (75/250) of examined samples with a prevalence of 32.9% (47/143) in cow calves and 26.2 % (28/107) in buffalo calves. The highest rate of infection was recorded in the diarrheic calves within one week (46.6%) and two weeks (41.4%) of age. Examined samples all over the four seasons of the years, 2013 and 2014 indicated the prevalence of Rotavirus antigen by 43.1% (56/130) during winter, 20% (5/25) during summer, 21.4% (12/56) during spring and 5.1% (2/39) during autumn. In conclusion, rotavirus was involved in the neonatal calves’ diarrhea, where its frequency was clearly higher in calves up to 2 weeks of life during winter season

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