You are in:Home/Publications/Isolation and molecular characterization of IBDV from Qualubyia governorate, Egypt, 2015

Prof. Ehab Mostafa El-Nahas Mohammed Nassar :: Publications:

Title:
Isolation and molecular characterization of IBDV from Qualubyia governorate, Egypt, 2015
Authors: Gabr F. El-Bagoury, Ehab M. El-Nahas, Ayman S. El-Habbaa
Year: 2015
Keywords: IBDV, Virus isolation, RT-PCR, VP2 Sequencing, phylogeny
Journal: BENHA VETERINARY MEDICAL JOURNAL
Volume: 28
Issue: 2
Pages: 283-294
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper Ehab Mostafa El-Nahas Mohammed Nassar _IBDV 2015.PDF
Supplementary materials Not Available
Abstract:

Infectious bursal disease virus (IBDV) remerged frequently in Qualubyia was the cause of serious economic losses. Twenty field samples were collected from broiler chick farms in different localities of the governorate in January 2015, subjected for isolation on specific pathogen free-embryonated chicken eggs (SPF-ECE), detection using AGID and RT-PCR. Ten samples showed positive results with egg isolation, AGID and RT-PCR. Sequence analysis and characterization of the variable region of VP2 gene purified from the PCR product showed many amino acid substitutions that may affect IBDV antigenicity and virulence. Comparative phylogenetic analysis based on the sequence of the hypervariable region of VP2 gene clustered the isolated IBDV at a distance from the other reference IBDV from Egypt, Europe and other vaccinal strains. It was concluded that substitution mutations observed with the hypervariable region of VP2 gene of the isolated IBDV could affect the virus antigenicity and further studies on the efficacy of vaccines used locally against this variant IBDV strain have to be investigated.

Google ScholarAcdemia.eduResearch GateLinkedinFacebookTwitterGoogle PlusYoutubeWordpressInstagramMendeleyZoteroEvernoteORCIDScopus