Lactic acid bacteria that grow as the adventitious microflora of foods or that are added to foods as cultures are
generally considered to be harmless or even an advantage for human health. The objective of this study was to
isolate, screen and identify lactic acid bacteria from different sources. As well, evaluate the more potent lactic
acid bacteria isolates for their probiotic characteristics. Ninety-six bacterial isolates were isolated from different
18 sources, 51 (53%) isolates were able to hydrolyze 0.5% bile salts while 28 isolates were able to hydrolyze 1%
bile salts. Also, only 11 isolates were able to tolerate 2% bile salts after 6h incubation periods. On the other hand,
lactic acid bacteria isolates were able to survive at pH 1.5 and 3.0 as well 8.0. Also, these isolates were potent for
cholesterol assimilation, NaCl tolerance, exopolysaccharides production, antioxidant activity. Lactic acid bacteria
isolate MS87 that exhibited excellent probiotic characteristics was identified using 16S rRNA sequence as
Pediococcus pentosaceus. This study led to suggest that environmental-derived lactic acid bacteria-strains could
be used as a potential source of probiotic.
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