Background: Claudin-1 And Ki 67 have been implicated in tumorigenesis in many cancers, but their significance in breast ductal carcinoma remains unclear. The aim of this study is to assess their possible role in different molecular subtypes of breast ductal carcinoma.
Methods: Immunohistochemistry was performed to examine the expression of Claudin-1 And Ki 67 in 60 cases of female breast ductal carcinoma (54 cases of invasive carcinoma of NST and 6 cases of DCIS) previously stained with (ER, PR, Her2). Six cases of apparently normal breast tissue adjacent to fibroadenoma were taken as control. Statistical analysis methods were used to evaluate the relationship between Claudin-1 and Ki 67 and various clinicopathological, immunopathological parameters and molecular subtypes.
Results: There is statistically significant correlation between claudin1 & ki67 expression and various clinicopathological, immunopathological parameters and molecular subtypes. High expression of both markers have direct significant correlation with poor prognostic factors as higher tumor grade, higher nuclear grade,large tumor size, positive lymph node metastasis, positive distant metastasis, high stage, positive LVI and poor NPI and negative significant statistical correlation with hormonal receptors. luminal B and TN patients have higher Ki-67 compared to other subtypes. TN & her2 enriched patients have higher claudin1 expression compared to other subtypes. All cases of luminal A have low expression of both ki67 and caludin1. while some cases of luminal B subtypes showed low expression of claudin1 and others showed high expression of claudin1
Conclusions: The results suggested that both ki67 and claudin1 may be considered a valuable biomarker in breast cancer patients and be used in diagnosis & prognosis of different molecular subtypes breast ductal carcinoma.
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