Background: Cyclophosphamide (CP), as an anticancer agent, causes ovarian toxicity and subsequent infertility in women. Aim of work: to determine the effect of BM-MSCs & AT-MSCs in the treatment of cyclophosphamide-induced ovarian damage in rats. Materials & Methods: Forty Female adult albino rats were randomly divided into four groups. Group I (control group). Group II (cyclophosphamide treated group) were subjected to intra-peritoneal injection of 150 mg/kg-B.W , followed by a dose of 8 mg/kg every week , the rats were scarified after 3 weeks . Group III (BM-MSCs treated group) were treated by cyclophosphamide in the same manner as in group II and after five weeks from the second CLP injection 100 μl of BM-MSCs mixture (2.5x106cells) in 100 µl PBS was injected intra-venous. Group IV(AT-MSCs treated group) were treated by CLP as in group II and after 5 weeks from the second CLP injection, 100 μl of AT-MSCs mixture (2.5x106cells) in 100µl PBS was injected intra-venous , Then 30 days after cell therapy the rats were scarified , Ovarian sections were subjected to H&E, Masson ́s Trichrome and Immunohistochemical staining for anti-PCNA antibody. The mean number of primordial follicles, mean area % of collagen fibers, mean area % of +ve immunoreactivity for PCNA were measured by histomorphometric studies and statistically analyzed. Results: Histological examination of the ovaries of CLP treated group revealed severe degeneration of ovarian follicles with sloughing of granulosa cells. The ovarian follicles treated with BM-MSCs showed a normal appearance of oogenesis in comparison to the cyclophosphamide-group. The AT-MSCs treated group rats showed relative improvement of oogenesis but no graafian follicle was detected. Conclusion: BM-MSCs and AT-MSCs were impressive in the treatment of ovarian failure in albino rat model where they restore the ovarian structure and protected ovary against CP-induced toxicity. However and BM-MSCs more effective. |
