Different Phages parasitizing Xanthomonas vesicatoria (the causal
agent of the bacterial spot disease of tomato) were isolated from infected
leaves of tomato and from tomato rhizosphere soil, using enrichment
technique. The phages produced plaques (4-5mm, diameter) with a distinct
translucent spreading halo. Presumptive phage particles associated with X
vesicatoria were observed by Transmission Electron Microscope (TEM).
Particle size and morphology of each phage isolate were examined by
electron microscopy. The obtained results indicated that, the isolated
phages were of the head and tail types. Four phages were detected and
designated A,B,C and D, their head diameters were found to be 57.83,
43.84, 63.43, and 79.25 nm, respectivelY,. Phages A and B were isolated
from tomato leaves, whereas, phages C and D were isolated from tomato
rhizosphere soils. This study seems to be the first record for phages of
Xanthomonas vesicatoria under the Egyptian conditions.
|