Background: Tramadol is a centrally acting analgesic drug. Chronic intake of Tramadol has negative impacts on testis. A polyphenol compounds such as Resveratrol which found in certain plants that has antioxidant and anti-inflammatory properties. Aim of the work: The target of this study to investigate the protective effects of Resveratrol on the testis of adult male albino rats against testicular toxicity induced by Tramadol. Materials and methods: Four experimental groups were served in this study. Each one included10 adult male albino rats as follows: Group I (control group): received 0.1 ml of physiological saline solution. Group II: received Resveratrol 20 mg/kg/day orally for 4 weeks. Group III: (Tramadol-treated group): received Tramadol 50 mg/kg/day orally for 4 weeks. GroupIV: received Tramadol 50 mg/kg body weight daily orally followed by Resveratrol 20 mg/kg body weight daily by intragastric gavage for 4 week. Serum levels of FSH, LH and testosterone have been estimated. Testicular specimens were processed for light microscopic examination and immunohistochemical studies and examined histologically. The data were collected from the experiment, recorded and analyzed using IBM SPSS Statistics software. Results: Tramadol-treated group showed severe distortion of the testicular structure in which many seminiferous tubules were degenerated with distorting their normal histological structure in addition, congested blood vessels in the interstitium can be observed. Meanwhile, sections of testes of group IV (Tramadol–Resveratrol treated group) showed nearly normal histology. Positive immune-reactivity for PCNA in the control, Resveratrol treated and Tramadol - Resveratrol treated groups while, Tramadol- treated group showed few PCNA positive cells. Serum LH, FSH and testosterone hormones levels were significantly higher in Tramadol-Resveratrol treated group compared to Tramadol treated group. Conclusion: Intake of Tramadol induced testicular damage which can be ameliorated by administration of Resveratrol in the concomitant with Tramadol treatment. |
