This study was conducted at the Laboratory, Greenhouse, and Farm of Faculty of Agriculture, Moshtohor, Banha University and the Virology Laboratory of Agricultural Genetic Engineering Institute, AGERI, Agric. Res. Center, ARC., Giza, Egypt, during two successive spring surveys (2003/2004 – 2004/2005). The aims of this study are surveyed the viruses affecting faba bean crop at Qalyoubia Governorate, identified the collected infected samples via ELISA, determine the disease incidence and severity. On the basis of specific virus symptoms, estimated the viruses frequencies, subsequently, determine the dominant faba bean viruses at the surveyed fields. Completely, isolated and identified the dominant virus using symptomatology, host range, mode of transmissions, physical properties, serological diagnosis, particle properties (inclusion bodies, via light microscope and virus-particle dimension via transmission electron microscope). Study the response of some cultivated faba bean cultivars to artificial infection with bean yellow mosaic virus under greenhouse conditions. Spraying faba bean crop with six systemic resistant inducers medicinal plant extracts as natural antiviral agents. Modified the serological reaction (SDS-double diffusion test) by replace agarose with Gelrite and sodium azide with Kombucha.
Key words: BYMV, Faba bean, Antiviral proteins, Cultivars, EM,Serology, Gelrite, Kombucha |
