Dr. Eman Shahwan Moheb El-Din Shahwan :: Publications:

The objectives of this study were isolation and identification of the most frequently and economically viruses causing serious losses in tomato crop in the different location of Qalyoubia Governorate, evaluating some medicinal plant extracts and kombucha filtrate as biotic inducers to induction systemic acquired resistance in the tomato plants against CMV and using more effective bioinducers as bioelicitors for control viruses infection via induction 'pathogenesis-related' (PR-1a) genes. Target virus was chosen according to its more frequently and severity among the isolated viruses in these locations at the winter season from the study year. Isolated virus was confirmed biologically and serologically assays. Extracts of two medicinal plants (Clerodendrum inerme L. Gaertn and Mirabilis jalapa L.) and were individually or in mixture in addition to kombucha filtrate were evaluated as bioinducers. All the four inducers were successfully in the induction of systemic acquire resistance (SAR) in the uninoculated tomato plants and sprayed with (50% v/v) of inducers. Tested bioinducers were used as biocontrol to inhibiting the virus infection of tomato plants as spraying every 15 days under greenhouse conditions. Pathogenesis-related (PR-1a) gene was molecularly isolated and identified via sequencer which compared with those recorded in the Gen-Bank. In conclusion, using medicinal extracts and other natural inducers were promise with good systemic acquired resistance against the great numbers of plant pathogens. In future, induction of resistance can be done cheaply and easily using natural substances.