To explore the inhibitory effect of two biotic inducers on tomato plant against Cucumber mosaic cucumovirus (CMV), salicylic acid (SA) activities were determined in the inoculated and non-inoculated tomato plants. Identify of CMV was confirmed by some differential hosts and dot blot immunoassay (DBIA) using polyclonal antibody specific CMV. Water extracts of Mirabilis jalapa, Clerodendrum inerme and Kombucha as antiviral to control the virus infection and detection systemic acquired resistance (SAR) were studied. SAR was detected by determination of salicylic acid (SA) resulted induction of protein related to biotic inducers, virus concentration and disease severity (DS). The obtained results from quantification of total SA in induced tomato plants (after 7 days of spraying inducers) showed high level of SA with Kombucha treatment followed by C. inerme and M. jalapa, while mixed (M. jalapa+C. inerme) gave the lowest level of SA compared with healthy and infected controls. On the other hand, after virus inoculation tomato plants showed reduction of virus and disease severity. M. jalapa, C. inerme and Kombucha showed lowest percentage of DS, while mixed M. jalapa+C. inerme showed high percentage of DS. Both of two biotics induced SA and inhibited CMV infection in the tomato plants.
Keywords: Plant defense, Antivirus, SA, Mirabilis jalapa, Clerodendrum inerme and Kombucha. |
