162 buffaloes of different ages were used in this investigation in Kaluobia Governorate, Egypt, during 2001. 31 (19.14%) out of them suffering from cutanous eruption in form of wart-like lesions (pedunculated or sessile) sited on different parts of the skin with or without some disturbance in appetite and general body condition.
Virological examination of warts suspension declared detection of papilloma virus after inoculation and passages into chicken embryos and cell culture respectively. Histopathologically, the cutanous warts showed extensive hyperkeratosis and acanthosis of the epidermis. Dermal fibroma and mononuclear inflammatory cellular infiltration of dermis were also noticed. The CAM inoculated with papilloma virus was hyperemic, edematous and showed multiple pinhead point foci and microscopically cleared marked hyperplasia of ectodermal cells with edema, hemorrhages and few inflammatory cellular infiltration of mesoderm were also found.
The virological and pathological findings beside clinical characteristic and predilection sites of the lesions on diseased animals proved field suspicion of the papillomatosis. Epidemiological data presented that, the incidence of the disease was more common in animals of less than 2 years age and it was higher during summer seasons. Treatment of the infected cases and suggested line of tackling the problem was traced.
Finally papilloma virus can infect Egyptian buffaloes with mild or moderate clinical forms. Further studies on molecular basis to differentiate between papilloma virus of buffaloes origin from that affecting cattle and the interspecies transmission criteria of the papilloma virus because these studies are of importance from the epidemiological point of view. Peculiar emphasis to prepare vaccine from local papilloma virus to be used under emergency statement in Egypt.
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