Mutagenesis is a highly efficient tool for establishing genetic variation and is widely used
for genetic enhancement in various plants. The key benefit of mutation breeding is the prospect of
enhancing one or several characteristics of a variety without altering the genetic background. In this
study, we exposed the seeds of Salvia officinalis to four concentrations of hydrazine hydrate (HZ),
i.e., (0%, 0.1%, 0.2%, and 0.3%) for 6 h. The contents of terpenoid compounds in the S. officinalis
plantlets driven from the HZ-treated seeds were determined by GC-MS, which resulted in the identification
of a total of 340 phytochemical compounds; 163 (87.48%), 145 (84.49%), 65 (97.45%), and
62 (98.32%), from the four concentrations of HZ (0%, 0.1%, 0.2%, and 0.3%), respectively. Furthermore,
we used the qRT-PCR system to disclose the “transcriptional control” for twelve TPS genes
related to terpenoid and terpene biosynthesis, namely, SoGPS, SoMYRS, SoNEOD, SoCINS, SoSABS,
SoLINS, SoFPPS, SoHUMS, SoTPS6, SoSQUS, SoGGPS, and SoGA2. Altogether, results are likely to
ensure some positive relationship between the concentrations of the chemical mutagen HZ used
for treating the seeds, the type and amount of the produced terpenes, and the expression of their
corresponding genes. |
