Root nodule formation in many leguminous plants is known to be affected by endogen
ous and exogenous factors that affect formation, development, and longevity of nodules in roots.
Therefore, it is important to understand the role of the genes which are involved in the regulation of
the nodulation signaling pathway. This study aimed to investigate the effect of terpenoids and terpene
biosynthesis genes on root nodule formation in Glycine max. The study aimed to clarify not only the
impact of over-expressing five terpene synthesis genes isolated from G. max and Salvia guaranitica
on soybean nodulation signaling pathway, but also on the strigolactones pathway. The obtained
results revealed that the over expression of GmFDPS, GmGGPPS, SgGPS, SgFPPS, and SgLINS genes
enhanced the root nodule numbers, fresh weight of nodules, root, and root length. Moreover, the
terpene content in the transgenic G. max hairy roots was estimated. The results explored that the
monoterpenes, sesquiterpenes and diterpenes were significantly increased in transgenic soybean
hairy roots in comparison with the control. Our results indicate the potential effects of terpenoids and
terpene synthesis genes on soybean root growth and nodulation. The study provides novel insights
for understanding the epistatic relationship between terpenoids, root development, and nodulation
in soybean. |
