One hundred blood samples (55 sheep and45 goats) (include 79 females and 21 males)were selected from
farms suffer from brucellosis and examined serologically by Buffered Acidified plate Antigen Test
(BAPAT), Rose Bengal plate Antigen test (RBPAT) , Tube Agglutination test (TAT), Complement Fixation
Test (CFT) and Immunochromatography assay (ICA). The results of BAPAT were (61.81%) and (73.33%),
RBPAT& TAT were (61.81%) and (66.66%) and CFT &ICA were (60%) and (66.66%) in sheep and goats
respectively for all tests. Moreover, the positive reactors in females among sheep and goats were (79.1%),
(76.56%), (81.25%), (80.95%) and (79.36%) using BAPAT, RBPAT, TAT, CFT and ICA respectively. The
sensitivity, specificity and agreement of BAPAT with CFT in sheep and goats were (92.06%, 75.67%, 86%)
respectively. The sensitivity, specificity and agreement of RBPT with CFT in sheep and goats were
(88.88%, 78.37%, 85%) respectively. The sensitivity, specificity and agreement of TAT with CFT in sheep
and goats were (84.12%, 70.27%, 79%) respectively. The sensitivity, specificity and agreement of ICA with
CFT in sheep and goats were (98.41%, 97.29%, 98%) respectively. The sensitivity, specificity of CFT was
100%. In conclusion the ICA proved to be the most accurate, cheapest, rapid and simplest test for diagnosis
of ovine brucellosis. |
