A total of 100 milk samples were collected from different animals (39 cows and 61 buffaloes) from El-
Kalyobia, El-Monofia and El-Sharkia Governorates. The bacteriological identification revealed that 42
Staphylococcus isolates (29/42 (69%) of Coagulase positive S.aureus and 13/42 (30.9%) of Coagulase
negative S.aureus) from subclinical mastitis and clinical mastitis 22/50 (44%) and 20/50 (40%)
respectively. PCR assay showed that clfA gene was detected in 10 isolates of S. aureus at 638bp. Also
four isolates were identified as Candida albicans from the same samples. Antimicrobial agents
sensitivity test revealed that 20 tested S.aureus isolates were resistant to Cefotaxime, Erythromycin,
Clindamycin, Penicillin, Amoxicillin/Clavulnic acid, Doxicyclin, Amoxicillin, Gentamycin and
Trimethoprim/Sulpha with different rates. The studying of antimicrobial activity of some essential oils
showed that MICs for Thyme, Lavander, Garlic, Cinamoon oils found to be effective against S. aureus
ranged from 80 - 2560, 80 - 2560, 5-640 and 5 - 5120 μg/ml respectively and MBCs ranged from 160
-2560, 20-2560, 20-1280 and 10-5120 μg/ml respectively. MIC50 was detected only for Thyme oil at
2560 μg/ml . Marjoram, Camphor and Tea oils did not have any effect against the tested strain .Candida
albicans isolates were resistant to antimicrobial agents and all tested essential oils. |
