Acute Renal Failure (ARF) has traditionally been defined as the
abrupt and progressive loss of kidney functions resulting in the retention of
urea and other nitrogenous waste products associated with interstitial
inflammation, tubular injury and increasing Tumor Necrosis Factor (TNF).
Mortality in patients with ARF remains high >50% in severely ill patients.
Klotho gene is a new anti-aging gene. Genetic mutation of klotho gene
causes multiple premature aging-like phenotypes and shortens lifespan.
Klotho gene is highly expressed in the kidney and a soluble form of klotho
functions as an endocrine substance that exerts multiple actions including
the modulation of renal solute transport and the protection of the kidney.
This study aimed to clarify the pre treatment and/or post treatment effect of
vitamin E as an antioxidant on kidney functions, klotho gene expression
and glutathione peroxidase-1 gene expression among rats with acute renal
failure. Using glycerol as oxidative stress factor to cause acute renal failure
and Real time PCR for assessment of gene expression of target gene in the
control and treated groups. Our results demonstrated that the vitamin E (α
tocopherol) as antioxidant factor decreased the kidney injuries as pre renal
failure administer and improve kidney function as post renal failure
administer. Those effects were through up regulating the Klotho as anti
aging gene and the Glutathione Peroxidase (GPx-1) as antioxidant gene
expression in the kidney tissue. We concluded that factors those up regulate
the klotho gene expression can use as protective factors against kidney
injuries and to improve kidney function in renal failure.