Background: transplantation of mesenchymal stem cells (MSCs)
provides an effective strategy to protect pancreatic tissue injury
in diabetes type 1. Many factors act as obstacles to their best
regenerative capacity. Aim of the work: To evaluate the role of
Adipose-derived mesenchymal stem cell (AD-MSCs) in the
treatment of type 1 DM and to compare their efficacy alone and
with a combination with resveratrol and long-acting insulin in the
pancreas of albino rats with streptozotocin induced T1DM.
Methods: 10 out of the 50 healthy adult male albino rats served
as donors for stem cells and the other 40 rats were subdivided
into two main groups: Group I (control group (16 rats), Group II
(STZ-diabetic group, (24) rats which were injected with a single
intraperitoneal injection of streptozotocin 50 mg/kg BW. This
group was subdivided into 4 groups. GII a: untreated rats, GII b:
received 1x10⁶ of cultured rat ADMSCs once intravenously into
the tail vein, GII c which was given MSCs plus resveratrol in
combination, GII d which was given MSCs & resveratrol and
insulin glargine. Pancreatic samples were prepared for immune
histochemical study, light and electron microscopic examination,
and morphometric studies. Results: The AD-MSCs-treated group
revealed moderate improvement in histological structure and
fasting blood glucose level. AD-MSCs, resveratrol and insulintreated group demonstrated higher improvement than that of the
MSCs treated group alone or dual combination of AD-MSCs plus
resveratrol. Conclusion: Better synergistic protective effect on
damaged pancreatic tissues can be observed in STZ induced DM
rats treated with AD- MSC coupling with resveratrol and insulin
treatment. |
